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高亮 博士

Liang Gao, Ph.D.


西湖大学是一片孕育着希望的土壤。感谢西湖大学为推动基础生命科学研究所做的努力和尝试。我们将以最好的研究成果来回报西湖大学以及公众和社会对我们研究的支持。西湖已至,未来可期。


一. 个人简介

高亮博士分别于2000年和2003年获清华大学学士和硕士学位,2009年获美国Purdue大学博士学位。2010年至2013年间在Howard Hughes医学研究院,Janelia研究所,诺贝尔奖获得者Eric Betzig 博士实验室从事博士后研究。2014年至2016年间任美国纽约州立大学石溪分校助理教授。2017至2018年间任美国3I显微镜公司首席研究科学家。2019年全职加入西湖大学。


二. 学术成果

(一)研究方向

我们目前的主要研究方向为开发用于生命科学研究的高速、高分辨率、活体三维荧光显微镜成像技术以及生物图像分析技术。同时我们也致力于使用这些技术探索和解释前沿生物问题。我们目前的研究工作主要包括以下三个方向。

1. 利用高速、高分辨率、三维成像技术和图像分析技术,使用线虫胚胎、斑马鱼胚胎和小鼠胚胎,建立用于定量研究细胞在三维组织中的行为的研究方法。

2. 高分辨率、高通量的三维组织成像技术。

3. 高通量、定量生物图像分析和解析技术。


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(二)研究亮点

Commercial Microscopes use our technology

·      Nuohai Life Science, LS18 light sheet microscope.

·      Intelligent Imaging Innovations, Inc., Cleared Tiling Lightsheet microscope.

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Nature Methods, “Tunable light sheet microscopy”, 2016, 6

An editor review about the tiling light sheet selective plane illumination microscopy (TLS-SPIM, patented) developed in my lab at the Stony Brook University. It is an effective light sheet microscopy technique enables low invasive 3D fluorescence imaging of multicellular specimens at high spatial and temporal resolution. The technique is under commercialization by multiple companies.

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Nature Methods, “Bessel beams beyond the limit”, 2013, 10

An editor review on the Bessel beam structured illumination microscopy technique I developed with Dr. Eric Betzig in the Janelia Research Campus of HHMI during my postdoctoral research. It is the first light sheet microscopy technique that allows high speed, live 3D fluorescence imaging beyond the diffraction limit.

 

Nature Methods, “Imaging life with thin sheets of light”, 2012, 9(1).

Nature, “Seeing cells with sheets of light”, 2011, 471, 268.

Nature Biotechnology, “Bessel beams for light relief”, 2011, 29(4).

BBC News, “Light sheets image life in 3D”, 03/07/2011.

HHMI News, “New microscope produces dazzling 3D movies of live cells”, March 04, 2011.

Editor reviews and news reports on the Bessel beam plane illumination microscopy technique I worked on with Dr. Planchon Thomas and Dr. Eric Betzig in the Janelia Research Campus of HHMI during my postdoctoral research. It is the first light sheet microscopy technique that allows low invasive, high speed 3D fluorescence imaging of biological processes at submicron isotropic 3D spatial resolution.

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DPAI used in the ExoMars rover “ROSALIND FRANKLIN”

Our invention, the discontinuous atmospheric pressure interface (DAPI), was used in the Mars Organic Molecule Analyzer (MOMA) of the latest ExoMars rover, “ROSALIND FRANKLIN”, to introduce ions from the Mars atmosphere to the mass analyzer vacuum chamber. The rover will be sent to the Mars in July 2020. (http://exploration.esa.int/mars/45103-rover-instruments)

 

Chemical & Engineering News / Science & Technology / Concentrates, “Interface boosts MS performance”, May 19, 2008, 86(20), 39-40.

A news report about a discontinuous atmospheric pressure interface (DAPI, patented) I invented in the Purdue University during my Ph.D. study. It allows efficient ion introduction from atmospheric pressure environments to high vacuum environments with very limited pumping capacities. 



(三)主要学术文章

1. Fu, Q., Martin, B., Matus, D., Gao, L. (2016) “Imaging multicellular specimens with real-time optimized tiling light sheet selective plane illumination microscopy”, Nature Communications, 7:11088.

2.  Gao, L.,* Shao, L., Chen, B.C., Betzig, E. (2014) “3D Live Fluorescence Imaging of Cellular Dynamics Using Bessel Beam Plane Illumination Microscopy”, Nature Protocols, 9, 1083-1011. (*Corresponding Author, Cover article)

3. Gao, L., Shao, L., Higgins, D.C., Poulton, J.S., Peifer, M., Davidson, M.D., Wu, X., Goldstein, B., and Betzig, E. (2012) “Noninvasive imaging of 3D dynamics in thickly fluorescent specimens beyond the diffraction limit”, Cell, 6, 1370-1385.

4. Planchon, T.A.,* Gao, L.,* Milkie, D.E., Davidson, M.W., Galbraith, J.A., Galbraith, C.G., Betzig, E. (2011) “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination”, Nature Methods, 8, 417-423. (*Equal Contribution, Cover article)

5. Gao, L., Cooks, R.G., Ouyang, Z. (2008) “Breaking the pumping speed barrier in mass spectrometry: Discontinuous Atmospheric Pressure Interface”, Analytical Chemistry, 80, 4026-4032.


(四)专利

1.Gao, L. and Chen, B.-C. “A method to tile lattice light sheets in lattice light sheet microscopes” (Provisional Patent filed)

2.Gao, L. (2015) “Method and apparatus for tiling light sheet selective plane illumination microscopy with real-time optimized light sheet”, US Patent pending (US2016/019143)

3.Ouyang, Z., Gao, L. Cooks, R.G., (2007) “Discontinuous Atmospheric Pressure Interface”, US Patent granted (US 8304718 B2)


三. 工作岗位

实验室现已开始招收博士研究生,并有科研助理、助理研究员和副研究员等不同级别的科研岗位。我们欢迎物理、光学、仪器、计算机、生物科学及相关专业,和我们有共同科研理念和热情的申请者。请希望进一步了解我们的科研工作和有兴趣加入我们的申请者和我们联系。联系方式: gaoliang@westlake.edu.cn


四. 学术文章

1. Gao, L.,* Tang, W.C., Tsai, Y.C., Chen, B.C.* (2019) “Lattice light sheet microscopy using tiling lattice light sheets”, Optics Express, 27(2), 1497-1506. (*Co Corresponding Author)

2. Jing, D., Zhang, S., Luo, W., Gao, X., Men, Y., Ma, C., Liu, X., Yi, Y., Bugde, A., Zhou, B.O., Zhao, Y., Yuan, Q., Feng, J.Q., Gao, L., Ge, W.P., Zhao, H. (2018) “Whole body tissue clearing and imaging with the PEG Associated Solvent System (PEGASOS)”, Cell Research, 28, 803-818.

3. Fu, Q., Martin, B., Matus, D., Gao, L., (2016) “Imaging multicellular specimens with real-time optimized tiling light sheet selective plane illumination microscopy”, Nature Communications, 7:11088.

4. Gao, L., (2015) “Extend the field of view of selective plane illumination microscopy by tiling the excitation light sheet”, Optics Express, 23(5), 6102-6111.

5. Gao, L., (2015) “Optimization of the excitation light sheet in selective plane illumination microscopy”, Biomedical Optics Express, 6(3), 881-890.

6. Gao, L.,* Shao, L., Chen, B.C., Betzig, E. (2014) “3D Live Fluorescence Imaging of Cellular Dynamics Using Bessel Beam Plane Illumination Microscopy”, Nature Protocols, 9, 1083-1011. (*Corresponding Author, Cover article)

7. Wang, D., Meza, D. Wang, Y. Gao, L., Liu, J.T.C. (2014) “Sheet-scanned dual axis confocal (SS-DAC) microscopy using Richardson-Lucy deconvolution”, Optics Letters, 39, 5431-5434

8. Gao, L., Shao, L., Higgins, D.C., Poulton, J.S., Peifer, M., Davidson, M.D., Wu, X., Goldstein, B., and Betzig, E. (2012) “Noninvasive imaging of 3D dynamics in thickly fluorescent specimens beyond the diffraction limit”, Cell, 6, 1370-1385.

9. Planchon, T.A.,* Gao, L.,* Milkie, D.E., Davidson, M.W., Galbraith, J.A., Galbraith, C.G., Betzig, E. (2011) “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination”, Nature Methods, 8, 417-423. (*Equal Contribution, Cover article)

10. Majoul, I., Gao, L., Eric Betzig, Onichtchouk, D., Butkevich, E., Kozlov, Y., Bukauskas, F., Bennett, M.V.L., Lippincott-Schwartz, J., Duden, R. (2013) “Fast structural responses of gap junction membrane domains to AB5 toxins”, Proceedings of the National Academy of Sciences of the USA, 110(44), E4125-33. 

11. Roh-Johnson, M., Shemer, G., Higgins, C.D., McClellan, J.H., Werts, A.D., TuLu, U.S., Gao, L., Betzig, E., Kiehart, D.P., Goldstein, B. (2012) “Triggering  a cell shape changes by exploiting pre-existing actomyosin constractions”, Science, 335, 1232-1235.

12. Legant, W.L., Choi, C.K., Miller, J.S., Shao, L., Gao, L., Betzig, E., Chen, C.S. (2012) “Multidimensional traction force microscopy reveals rotational moments about focal adhesions”, Proceedings of the National Academy of Sciences of the USA, 110(3), 881-886.

13. Thievessen, I., Fakhri, N., Steinwachs, J., Kraus, V., Mclsaac, R.S., Gao, L., Chen, B.C., Baird, M.A., Davidson, M.W., Betzig, E., Oldenbourg, R., Waterman, C.M., Fabry, B. (2015) “Vinculin is required for cell polarization, migration, and extracellular matrix remodeling in 3D collagen”, FASEB Journal, 14-268235.

14. Ritter, A.T., Asano, Y., Stinchcombe, J.C., Dieckmann, N.M.G., Chen, B.C., Gawden-Bone, C., van Engelenburg, S., Legant, W., Gao, L., Davidson, M.W., Betzig, E., Lippincott-Schwartz, J., Griffitys, G.M. (2015) “Actin depletion initiates events leading to granule secretion at the immunological synapse”, Immunity, 42(5), 864-876.

15. Gao, L., Li, G., Cooks, R.G. (2010) “Axial CID and high pressure resonance CID in a miniature ion trap mass spectrometer using a discontinuous atmospheric pressure interface”, Journal of The American Society for Mass Spectrometry, 21, 209-214.

16. Gao, L., Li, G., Cyriac, J., Nie, Z., Cooks, R.G. (2010) “Imaging of surface charge and the mechanism of desorption electrospray ionization mass spectrometry”, Journal of Physical Chemistry C, 114, 5331-5337.

17. Gao, L., Li, G., Nie, Z., Duncan, J., Ouyang, Z., Cooks, R.G. (2009) “Characterization of a discontinuous atmospheric pressure interface. Multiple ion introduction pulses for improved performance”, International Journal of Mass Spectrometry, 283, 30-34.

18. Gao, L., Sugiarto, A., Harper, J.D., Cooks, R.G., Ouyang, Z. (2008) “Design and characterization of a multisource hand-held mass spectrometer”, Analytical Chemistry, 80, 7196-7205. (Accelerated Article, Cover article)

19. Gao, L., Cooks, R.G., Ouyang, Z. (2008) “Breaking the pumping speed barrier in mass spectrometry: Discontinuous Atmospheric Pressure Interface”, Analytical Chemistry, 80, 4026-4032.

20. Gao, L., Song, Q., Noll, R.J., Duncan, J., Cooks, R.G., Ouyang, Z. (2007) “Glow discharge electron impact ionization source for miniature mass spectrometers”, Journal of Mass Spectrometry, 42, 675-680.

21. Gao, L., Song, Q., Patterson, G.E., Cooks, R.G., Ouyang, Z., (2006) “Handheld rectilinear ion trap mass spectrometer”, Analytical Chemistry, 78, 5994-6002. (Accelerated Article)

22. Cyriac, J., Wleklinski, M., Li, G., Gao, L., Cooks, R.G. (2012) “In situ Raman spectroscopy of surfaces by ion soft landing”, Analyst, 137, 1363-1369.

23. Li, G., Cyriac, J., Gao, L., Cooks, R.G. (2011) “Molecular ion yield enhancement in static secondary ion mass spectrometry by soft landing of protonated water clusters”, Surface and Interface Analysis, 43, 498-501.

24. Clare, A.T., Gao, L., Brkić, B., Paul R. Chalker, Taylor, S. (2010) “Linear ion trap fabricated using rapid manufacturing technology”, Journal of The American Society for Mass Spectrometry, 21, 317-322.

25. Song, Q., Xu, W., Smith, S.A., Gao, L., Chappell, W.J., Cooks, R.G., Ouyang, Z. (2010) “Ion trap mass analysis at high pressure: an experimental characterization”, Journal of Mass Spectrometry, 45, 26-34.

26. Huang, G., Gao, L., Duncan, J., Harper, J.D., Sanders, N.L., Ouyang, Z., Cooks, R.G. (2010) “Direct detection of benzene, toluene, and ethylbenzene at trace levels in ambient air by atmospheric pressure chemical ionization Using a handheld mass spectrometer”, Journal of The American Society for Mass Spectrometry, 21, 132-135.

27. Nie, Z., Guangtao Li, Goodwin, M., Gao, L., Cyriac, J., Cooks, R.G. (2009) “In situ SIMS Analysis and Reactions of Surfaces Prepared by Soft-Landing of Mass-selected Cations and Anions using an Ion Trap Mass Spectrometer”, Journal of American Society for Mass Spectrometry, 20, 949-956.

28. Song, Q., Smith, S.A., Gao, L., Xu, W., Volny, M., Ouyang, Z., Cooks, R.G. (2009) “Mass Selection of Ions from Beams Using Waveform Isolation”, Analytical Chemistry, 81, 1833-1840.

29. Keil, A., Hernandez-Soto, H., Noll, R.J., Fico, M., Gao, L., Ouyang, Z., Cooks, R.G. (2008) “Monitoring of toxic compounds in air using a handheld rectilinear mass spectrometer”, Analytical Chemistry, 80, 734-741.

30. Keil, A., Talaty, N., Janfelt, C., Noll, R.J., Gao, L., Ouyang, Z., Cooks, R.G. (2007) “Ambient mass spectrometry with a handheld mass spectrometer at high pressure”, Analytical Chemistry, 42, 675-680.

31. Ouyang, Z., Gao, L., Fico, M., Chappell, W.J., Noll, R.J., Cooks, R.G. (2007) “Quadrupole ion traps and trap arrays: geometry, material, scale, performance”, European Journal of Mass Spectrometry, 13, 13-18.